ANTH domain

The ANTH domain is a membrane binding domain that shows weak specificity for PtdIns(4,5)P2. It was found in AP180 (homologous to CALM^[1]) endocytotic accessory protein that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.^[2][3]

SymbolANTH

PfamPF07651

InterProIPR011417

OPM superfamily38

Quick facts Identifiers, Symbol ...

ANTH domain
Clathrin assembly lymphoid myeloid leukemia (CALM) protein
Identifiers
Symbol	ANTH
Pfam	PF07651
InterPro	IPR011417
OPM superfamily	38
OPM protein	1hfa
CDD	cd03564
Available protein structures: PDB   IPR011417 PF07651 (ECOD; PDBsum)   AlphaFold IPR011417 PF07651

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Its structure is a solenoid of 9 helices. The PtdIns(4,5)P2 binding residues are spread over several helices at the tip of the structure. The PtdIns(4,5)P2 binding sequence is Kx9Kx(K/R)(H/Y).

An ANTH domain is also found in HIP1 and HIP1R, and the PtdIns(4,5)P2 binding sequence is conserved.