Middlebrook 7H10 Agar
Growth medium used to culture Mycobacterium species
From Wikipedia, the free encyclopedia
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.[1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition
Ingredients (g/L)[2]
- Ammonium sulfate, 0.50
- Monopotassium phosphate, 1.50
- Disodium phosphate, 1.50
- Sodium citrate, 0.40
- Magnesium sulfate, 0.025
- Zinc sulfate, 0.001
- Copper sulfate, 0.001
- L-Glutamic acid, 0.50
- Ferric ammonium citrate, 0.04
- Pyridoxine hydrochloride, 0.001
- Biotin, 0.0005
- Malachite green, 0.00025
- Agar, 15.00
- Calcium chloride, 0.0005
Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.