RMND5B
Protein-coding gene in the species Homo sapiens
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Required for meiotic nuclear division 5 homolog B (S. cerevisiae), also known as RMND5B, is a protein which in humans is encoded by the RMND5B gene.[5] It has a zinc finger domain and is highly conserved throughout many eukaryotic organisms.
| RMND5B | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Aliases | RMND5B, GID2, GID2B, required for meiotic nuclear division 5 homolog B | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 1913339; HomoloGene: 100777; GeneCards: RMND5B; OMA:RMND5B - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Protein sequence
This protein is rich in leucine (14.0%) and might belong to the protein family of leucine-rich repeats
1 meqcacvere ldkvlqkflt ygqhcersle ellhyvgqlr aelasaalqg tplsatlslv
61 msqccrkikd tvqklasdhk dihssvsrvg kaidrnfdse icgvvsdavw dareqqqqil
121 qmaivehlyq qgmlsvaeel cqestlnvdl dfkqpfleln rilealheqd lgpalewavs
181 hrqrllelns slefklhrlh firllaggpa kqlealsyar hfqpfarlhq reiqvmmgsl
241 vylrlgleks pychlldssh waeicetftr dacsllglsv esplsvsfas gcvalpvlmn
301 ikavieqrqc tgvwnhkdel pieielgmkc wyhsvfacpi lrqqtsdsnp piklicghvi
361 srdalnklin ggklkcpycp meqnpadgkr iif
Homology
CAD28476 is highly conserved in many eukaryotic organism. Its high conservation suggests that it plays a primary role in meiosis.
Orthologs
| taxonomic name | common name | NCBI entry | Percentage of sequence similarity | Length (AAs) | comments |
|---|---|---|---|---|---|
| Homo sapiens | Human | 100% | 393 | hypothetical Protein for Meiosis | |
| Pan troglodytes | Chimpanzee | 99.7% | 393 | required for meiotic nuclear division 5 homolog B isoform 6 | |
| Macaca mulatta | Rhesus macaque | 98.5% | 393 | Similar to CG3295-PA isoform 11 | |
| Rattus norvegicus | Norway rat | 98.2% | 393 | Similar to RIKEN cDNA 0610039K22 | |
| Mus musculus | House mouse | 97.7% | 393 | Required for meiotic nuclear division 5 homolog B | |
| Equus caballus | Horse | 97.7% | 273 | PREDICTED: similar required for meiotic nuclear division 5 homolog B | |
| Bos taurus | Cattle | 95.4% | 393 | required for meiotic nuclear division 5 homolog B | |
| Danio rerio | Zebrafish | 72.8% | 391 | required for meiotic nuclear division 5 homolog B | |
| Xenopus laevis | African clawed frog | 70% | 391 | MGC84431 protein | |
| Canis lupus familiaris | Dog | 70% | 391 | PREDICTED: similar to CG3295-PA isoform A | |
| Tetraodon nigroviridis | Spotted green pufferfish | 68.5% | 417 | unnamed protein product | |
| Ornithorhynchus anatinus | Platypus | 64.5% | 389 | ||
| Branchiostoma floridae | Florida lancelet | 57% | 491 | hypothetical protein BRAFLDRAFT_126901 | |
| Nematostella vectensis | Sea anemone | 50.1% | 389 | ||
| Culex quinquefasciatus | Southern house mosquito | 48.5% | 392 | conserved hypothetical protein | |
| Aedes aegypti | Yellow fever mosquito | 48.2% | 392 | hypothetical protein AaeL_AAEL009407 | |
| Strongylocentrotus purpuratus | Purple urchin | 48.2 | 405 | PREDICTED: Similar to MGC88921 protein | |
| Drosophila virilis | Fruit fly | 41.5% | 437 | GJ20343 | |
| Drosophila melanogaster | Fruit fly | 40.3% | 431 | CG3295 | |
| Acyrthosiphon pisum | Acrythosiphon | 39.5% | 366 | PREDICTED: required for meiothic nuclear division 5 homolg A | |
| Oryza sativa | Rice | 32.2% | 386 | membrane protein-like | |
Zinc finger domain
Two domains were predicted by the program BLIMPS[6] to exist in the protein of which one of the domains contains a zinc finger domain.
Zinc finger domains assist the binding of the protein to nucleic acids. This points to a direct interaction of CAD28476 with DNA during meiosis. By comparing CAD28476 with a related zinc finger protein[7] in a local sequence alignment using LALIGN,[8] the amino acids His359, Cys381 and Cys384 could be attributed to the zinc finger domain. This zinc finger structure is uncommon in the way that it involves one histidine instead of two.
Expression
Microarray data show that CAD28476 is highly expressed in tissue where meiosis occurs like in testis and ovaries. Moreover, it is also highly expressed in the brain around the hypothalamus.
Transcriptional regulation
The analysis of the promoter region (tools on the page rVista .[9] were used) shows that there are several transcription factor binding sites localized in conserved regions . It is very likely that the transcription factor Ets-1 which belongs to the ETS transcription factor family and its core binding factor CBF are involved in regulation of transcription since they both have independent binding sites.
Interacting proteins
There were two proteins predicted[10] which interact with CAD28476.
| name of the protein | Uniprot entry | information about the gene |
|---|---|---|
| Ewing sarcoma breakpoint region 1, isoform CRA_a | gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. | |
| Mothers against decapentaplegic homolog 4 | SMAD4 |