2000 Empress's Cup

It was contested by 19 teams, and Nippon TV Beleza won the championship.^[1]

• Seiwa Gakuen High School 1-0 Kamimura Gakuen High School
• Sapporo Linda 0-2 Shimizudaihachi SC
• Saibi High School 0-7 Nippon Sport Science University

• Nippon TV Beleza 8-0 Seiwa Gakuen High School
• Renaissance Kumamoto FC 0-3 JEF United Ichihara
• Speranza FC Takatsuki 7-0 Nawashiro Ladies
• YKK Tohoku LSC Flappers 5-0 Nippon TV Menina
• Tasaki Perule FC 10-1 Shimizudaihachi SC
• Osaka University of Health and Sport Sciences 0-1 Urawa Reinas FC
• Takarazuka Bunnys 3-0 Scramble FC
• Nippon Sport Science University 0-3 Iga FC Kunoichi

• Nippon TV Beleza 3-0 JEF United Ichihara
• Speranza FC Takatsuki 2-1 YKK Tohoku LSC Flappers
• Tasaki Perule FC 4-0 Urawa Reinas FC
• Takarazuka Bunnys 0-4 Iga FC Kunoichi

• Nippon TV Beleza 5-1 Speranza FC Takatsuki
• Tasaki Perule FC 1-0 Iga FC Kunoichi

• Nippon TV Beleza 2-1 Tasaki Perule FC

Nippon TV Beleza won the championship.