2001 Empress's Cup

It was contested by 20 teams, and Iga FC Kunoichi won the championship.^[1]

• Fujimi FC Angels 1-2 Hokkaido Bunkyo University Meisei High School
• Ohara Gakuen JaSRA 2-1 Tokyo Women's College of Physical Education
• Saibi High School 0-6 Kyoto Shiko SC
• Fukuoka Jogakuin FC Anclas 1-3 Seiwa Gakuen High School

• Nippon TV Beleza 12-0 Hokkaido Bunkyo University Meisei High School
• JEF United Ichihara 1-2 Takarazuka Bunnys
• Urawa Reinas FC 5-0 Hiroshima Minami High School
• Ohara Gakuen JaSRA 0-6 Iga FC Kunoichi
• YKK Tohoku LSC Flappers 3-0 Kyoto Shiko SC
• AS Elfen FC 0-3 Shimizudaihachi SC
• Speranza FC Takatsuki 4-0 Renaissance Kumamoto FC
• Seiwa Gakuen High School 0-10 Tasaki Perule FC

• Nippon TV Beleza 4-0 Takarazuka Bunnys
• Urawa Reinas FC 0-1 Iga FC Kunoichi
• YKK Tohoku LSC Flappers 4-1 Shimizudaihachi SC
• Speranza FC Takatsuki 0-3 Tasaki Perule FC

• Nippon TV Beleza 1-2 Iga FC Kunoichi
• YKK Tohoku LSC Flappers 1-3 Tasaki Perule FC

• Iga FC Kunoichi 2-1 Tasaki Perule FC

Iga FC Kunoichi won the championship.