Aliphatic nitrilase
Class of enzymes
From Wikipedia, the free encyclopedia
In enzymology, an aliphatic nitrilase also known as aliphatic nitrile aminohydrolase (EC 3.5.5.7) is an enzyme that catalyzes the hydrolysis of nitriles to carboxylic acids:
- R-CN + 2 H2O R-COOH + NH3
| aliphatic nitrilase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.5.5.7 | ||||||||
| CAS no. | 9024-90-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are an aliphatic nitrile (R-CN) and H2O, whereas its two products are a carboxylic acid (R-COOH) and NH3.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in nitriles. This enzyme participates in styrene degradation.
References
* Kobayashi M, Yanaka N, Nagasawa T, Yamada H (1992). "Primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from Rhodococcus rhodochrous K22 and expression of its gene and identification of its active site residue". Biochemistry. 31 (37): 9000–7. doi:10.1021/bi00152a042. PMID 1390687.
- Pace HC, Brenner C (2001). "The nitrilase superfamily: classification, structure and function". Genome Biol. 2 (1) REVIEWS0001. doi:10.1186/gb-2001-2-1-reviews0001. PMC 150437. PMID 11380987.
 | This EC 3.5 enzyme-related article is a stub. You can help Wikipedia by adding missing information. |