The tram route formed a half-circle from the south of Brussels to its north via the eastern part of the city. It used to start at Brussels-South railway station, where Eurostar and Thalys trains from the United Kingdom and France arrive, but the route was later shortened to start at the Vanderkindere stop. Tram route 24 followed the same rails as tram 23 between the Vanderkindere and Princesse Elisabeth/Prinses Elisabeth stops, where route 24 deviated towards Schaerbeek railway station, one stop further. Tram route 25 also followed the same rails as routes 23 and 24 for long, between the Buyl and Meiser stops.
From 14 March 2011, the newly formed tram 7 follows the same route as tram 23, but with a much higher frequency. Because of the frequency being brought up to 'metro' level, its line number was altered to the lower regions, for those are the metro and so-called 'chrono' tramlines. Tram 24 was also taken over completely by the newly formed tram 7.