Ham's tissue culture medium
From Wikipedia, the free encyclopedia
Growth medium for mammalian cells
Ham's tissue culture medium is a growth medium for mammalian cells.[1]
It contains in amounts dissolved in 1 liter of triple distilled water:
- L-Arginine 211 mg
- Biotin 0.024 mg
- L-Histidine 21 mg
- Calcium pantothenate 0.7 mg
- L-Lysine 29.3 mg
- Choline chloride 0.69 mg
- L-Methionine 4.48 mg
- i-inositol 0.54 mg
- L-Phenylalanine 4.96 mg
- Niacinamide 0.6 mg
- L-Tryptophan 0.6 mg
- Pyridoxine hydrochloride 0.2 mg
- L-Tyrosine 1.81 mg
- Riboflavin 0.37 mg
- L-Alanine 8.91 mg
- Thymidine 0.7 mg
- Glycine 7.51 mg
- Cyanocobalamin 1.3 mg
- L-Serine 10.5 mg
- Sodium pyruvate 110 mg
- L-Threonine 3.57 mg
- Lipoic acid 0.2 mg
- L-Aspartic acid 13.3 mg
- CaCl2 44 mg
- L-Glutamic acid 14.7 mg
- MgSO4·7H2O 153 mg
- L-Asparagine 15 mg
- Glucose 1.1 g
- L-Glutamine 146.2 mg
- NaCl 7.4 g
- L-Isoleucine 2.6 mg
- KCl 285 mg
- L-Leucine 13.1 mg
- Na2HPO4 290 mg
- L-Proline 11.5 mg
- KH2PO4 83 mg
- L-Valine 3.5 mg
- Phenol red 1.2 mg
- L-Cysteine 31.5 mg
- FeSO4 0.83 mg
- Thiamine hydrochloride 1 mg
- CuSO4·5H2O 0.0025 mg
- Hypoxanthine 4 mg
- ZnSO4·7H2O 0.028 mg
- Folic acid 1.3 mg
- NaHCO3 1.2 g
See also
- Biotechnology
- Brewing
- Cell biology
- DMEM Dulbecco's Modified Eagle's Medium
- Eagle's minimal essential medium (a minimum ingredient cell culture medium)
- Fermentation (food)
- GMEM (Glasgow's Minimal Essential Medium)
- Murashige and Skoog medium (Plant cell culture medium)
- RPMI (Roswell Park Memorial Institute medium), for lymph cells
References
- ↑ Shenoy, Manjula (2007). Animal Biotechnology. Firewall Media. pp. 19–20. ISBN 978-81-318-0108-6.