| Organization | Year founded | Focus | Status | Refs |
|---|
| Accord Alliance | 2008 | Healthcare | Active |
| ACT UP | 1987 | HIV/AIDS | Active |
| Advocates for Informed Choice | 2006 | Intersex | Renamed interACT |
| Affirmation: LGBTQ Mormons, Families & Friends | 1977 | Mormons | Active |
| Against Equality | 2009 | Anti-capitalist discourse | |
| American Foundation for Equal Rights | 2009 | Prop. 8 opposition | Active |
| American Veterans for Equal Rights | 1990 | Veterans | Active |
| American Military Partner Association | 2009 | LGBTQ military families and Veterans | Merged with OutServe-SLDN and renamed to Modern Military Association of America in 2019 |
| Aromantic-spectrum Union for Recognition, Education, and Advocacy | 2019 | Aromanticism | Active |
| Asexual Visibility and Education Network | 2001 | Asexuality | Active |
| Athlete Ally | 2011 | Sports | Active |
| Atticus Circle | 2004 | Straight allies | Active |
| Bash Back! | 2007 | Anarchist direct action | Dissolved |
| Bialogue | 2005 | Bisexuality | |
| BiNet USA | 1990 | Bisexuality | Active |
| Bisexual Resource Center | 1985 | Bisexuality | Active |
| Campus Pride | 2001 | Students | Active |
| Cheer, Dorothy, Cheer! | 2002 | Awareness | |
| COLAGE | 1990 | Children of LGBTQ parents | Active |
| Daughters of Bilitis | 1955 | Lesbian | Dissolved |
| El/La Para TransLatinas | 2006 | Latinas who are trans women | Active |
| Empowering Spirits Foundation | 2008 | Community service | Dissolved |
| Equality Across America | 2009 | National Equality March | Dissolved |
| Equality Federation | 1997 | | Active |
| Family Equality Council | 1979 | | |
| Fight OUT Loud | 2007 | | |
| Fine By Me | 2003 | | Merged into Atticus Circle |
| Freedom to Marry | 2003 | Marriage equality | Dissolved |
| Gay, Lesbian and Straight Education Network (GLSEN) | 1990 | | Active | |
| Gay Liberation Front#United States (GLF) | 1969 | | Dissolved |
| Gay Liberation Network | 1998 | Direct action | Active |
| Gay Rights National Lobby | 1976 | | Merged into the HRC |
| GenderPAC (GPAC) | 1995 | | Dissolved |
| GetEQUAL | 2010 | | Active |
| GLAAD | 1985 | | Active |
| GLBTQ Legal Advocates & Defenders (GLAD) | 1978 | | |
| GLIFAA | 1992 | | Active |
| GLMA: Health Professionals Advancing LGBTQ Equality | 1994
(1981) | Homophobia in medicine | Active |
| Global Equality Fund | 2011 | | |
| GSA Network | 1998 | | Active |
| Human Rights Campaign (HRC) | 1980 | | Active |
| Immigration Equality | 1994 | | Active |
| interACT | 2006 | | Active |
| Integrity USA | 1974 | | |
| International Foundation for Gender Education (IFGE) | 1987 | | |
| Intersex Campaign for Equality (IC4E) formerly OII-USA | 2011 | | |
| Join the Impact | 2008 | | |
| Keshet | 2001 | | Active |
| Lambda Legal | 1971 | | Active |
| Lavender Menace | 1970 | | |
| Lesbian and Gay Band Association | 1982 | | |
| Lesbian Avengers | 1992 | | |
| LGBTQ Victory Fund | 1991 | | |
| Love Makes a Family | 1992 | | Dissolved |
| LPAC | 2012 | | Active |
| Marriage Equality USA | 1998 | | Dissolved |
| Mattachine Society | 1950 | | |
| Matthew Shepard Foundation | 1998 | | Active |
| Modern Military Association of America | 2012 | LGBTQ military, military families, and veterans | Active—Renamed from OutServe-SLDN with the merger of American Military Partner Association |
| National Black Justice Collective (NBJC) | 2003 | | Active |
| National Center for Lesbian Rights (NCLR) | 1977 | | Active |
| National Center for Transgender Equality (NCTE) | 2003 | | Active |
| National Coalition of Black Lesbians and Gays | 1978 | | Dissolved |
| National Gay Pilots Association (NGPA) | 1990 | | Active |
| National LGBT Chamber of Commerce (NGLCC) | 2002 | | Active |
| National Lesbian and Gay Journalists Association (NLGJA) | 1990 | | Active |
| National LGBTQ Task Force (The Task Force) | 1973 | | Active |
| National Transgender Advocacy Coalition | 1999 | | |
| NOH8 Campaign | 2009 | Prop. 8 | |
| North American Conference of Homophile Organizations (NACHO) | 1966 | | Dissolved in 1970 |
| ONE National Gay & Lesbian Archives | 1952 | | |
| Out & Equal | 1996 | | Active |
| OutServe-SLDN | 2012 | LGBTQ military and Veterans | Merged with American Military Partner Association and renamed Modern Military Association of America in 2019 |
| PFLAG | 1973 | | Active |
| Pride at Work | 1994 | | Active |
| Queer Nation | 1990 | | |
| RESYST | 2000 | | Dissolved |
| Rainbow Railroad USA | 2015 | Global LGBTQI+ refugee support and advocacy | Active |
| Rainbow Sash | 1998 | | |
| Services & Advocacy for GLBT Elders (SAGE) | 1979 | | Active |
| Servicemembers Legal Defense Network (SLDN) | 1993 | | Merged into OutServe-SLDN |
| Society for Human Rights | 1924 | | Dissolved |
| Soulforce | 1998 | | |
| Sports Equality Foundation | 2023 | LGBTQ Representation in Sport & the LGBTQ Sports Hall of Fame | Active |
| StartOut | 2009 | | Active |
| Sylvia Rivera Law Project | 2002 | Legal aid | Active |
| The Ace and Aro Advocacy Project (TAAAP) | 2018 | Aromanticism
Asexuality | Active |
| Think Again | 1997 | | Dissolved |
| Trans Student Educational Resources | 2011 | | Active |
| Transgender Law Center | 2002 | | Active |
| Truth Wins Out | 2006 | | Active |