2020 Internazionali di Tennis Città di Trieste

Country	Player	Rank1	Seed
AUS	Alexei Popyrin	103	1
FRA	Antoine Hoang	136	2
GER	Yannick Hanfmann	143	3
ITA	Lorenzo Giustino	153	4
ITA	Roberto Marcora	158	5
ITA	Alessandro Giannessi	160	6
AUT	Jurij Rodionov	166	7
SVK	Lukáš Lacko	172	8

• ¹ Rankings are as of 16 March 2020.^[1]

The following players received wildcards into the singles main draw:

• Matteo Gigante
• Lorenzo Musetti
• Giulio Zeppieri

The following player received entry into the singles main draw using a protected ranking:

• Maximilian Marterer

The following players received entry from the qualifying draw:

• Carlos Alcaraz
• Geoffrey Blancaneaux
• Riccardo Bonadio
• Tomás Martín Etcheverry

The following players received entry as lucky losers:

• Viktor Galović
• Tobias Kamke

• Carlos Alcaraz def. Riccardo Bonadio 6–4, 6–3.

• Ariel Behar / Andrey Golubev def. Hugo Gaston / Tristan Lamasine 6–4, 6–2.