2026 Challenger de Santiago

Country	Player	Rank1	Seed
PAR	Daniel Vallejo	104	1
NED	Guy den Ouden	156	2
POR	Jaime Faria	163	3
POR	Henrique Rocha	169	4
ARG	Alex Barrena	179	5
COL	Daniel Elahi Galán	182	6
ARG	Juan Pablo Ficovich	186	7
BRA	João Lucas Reis da Silva	205	8

• ¹ Rankings are as of 2 March 2026.^[1]

The following players received wildcards into the singles main draw:

• Diego Dedura
• Bastián Malla
• Amador Salazar

The following players received entry into the singles main draw as alternates:

• Daniel Dutra da Silva
• Facundo Mena

The following players received entry from the qualifying draw:

• Cannon Kingsley
• Igor Marcondes
• Nicolas Moreno de Alboran
• Pedro Sakamoto
• Benjamín Torrealba
• Gonzalo Villanueva

The following player received entry as a lucky loser:

• Guido Iván Justo

• Genaro Alberto Olivieri def. Henrique Rocha 6–4, 6–4.

• Gianluca Cadenasso / Paulo André Saraiva dos Santos def. Miguel Ángel Reyes-Varela / Federico Zeballos 6–3, 7–5.