Asparagine synthase (glutamine-hydrolysing)
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Asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4, asparagine synthetase (glutamine-hydrolysing), glutamine-dependent asparagine synthetase, asparagine synthetase B, AS, AS-B) is an enzyme with systematic name L-aspartate:L-glutamine amido-ligase (AMP-forming).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
- ATP + L-aspartate + L-glutamine + H2O AMP + diphosphate + L-asparagine + L-glutamate (overall reaction)
- (1a) L-glutamine + H2O L-glutamate + NH3
- (1b) ATP + L-aspartate + NH3 AMP + diphosphate + L-asparagine
| Asparagine synthase (glutamine-hydrolysing) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Asparagine synthetase B dimer, E.Coli | |||||||||
| Identifiers | |||||||||
| EC no. | 6.3.5.4 | ||||||||
| CAS no. | 37318-72-2 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
The enzyme from Escherichia coli has two active sites.[6]
