Cloacibacillus evryensis
From Wikipedia, the free encyclopedia
| Cloacibacillus evryensis | |
|---|---|
Scientific classification  | |
| Domain: | Bacteria |
| Kingdom: | Thermotogati |
| Phylum: | Synergistota |
| Class: | Synergistia |
| Order: | Synergistales |
| Family: | Synergistaceae |
| Genus: | Cloacibacillus |
| Species: | C. evryensis |
| Binomial name | |
| Cloacibacillus evryensis Ganesan et al. 2008[1] | |
| Type strain | |
| strain 158, DSM 19522, JCM 14828[2][3] | |
Cloacibacillus evryensis is a Gram-negative, anaerobic, mesophilic, rod-shaped and non-motile bacterium from the genus of Cloacibacillus which has been isolated from sewage sludge from a wastewater treatment plant in Évry, France.[1][3][4][5][6]
Cloacibacillus evryensis is part of the genus Cloacibacillus, and was first proposed and published in 2008.[4] There are 12 genera in the phylum: Aminiphilus, Aminobacterium, Aminomonas, Anaerobaculum, Cloacibacillus, Dethiosulfovibrio, Fretibacterium, Jonquetella, Pyramidobacter, Synergistes, Thermanaerovibrio, and Thermovirga.[7] The only other described neighbor within the genus is C. porcorum which was discovered in the intestinal tract of a pig.[8] A genome comparison has found a 90% similarity between the two species.[8]
Discovery
Cloacibacillus evryensis was discovered in a wastewater treatment plant in Évry, France and first described by researchers on September 1, 2008.[6] The specific location of the isolate was a mesophilic anaerobic digester with working parameters of 33 °C, a pH of 7.2 and a retention time of 37.5 days.[6]
Isolation
Isolation was done using the Hungate technique with an enrichment medium containing contents such as KH2PO4, K2HPO4, NaCl, SO4, sodium citrate, yeast extract, Casamino acids and trace elements.[6] After inoculation of the sample, the enrichments were incubated at 33 °C in anaerobic conditions and transferred periodically onto fresh medium.[6] Growth was detected after 5-7 days and another isolation of the sample was inoculated onto a fresh enrichment medium and grown in anaerobic jars at 33 °C and 2 atm pressure.[6] Cloacibacillus evryensis was one of the purified strains that was isolated from the sample.
Morphology
Cloacibacillus evryensis is a Gram-negative nonmotile organism with rod shaped cells that are commonly seen as diplococci.[6][9] The rod-shaped bacterium is usually 2.0-3.0 μm by 0.8-1.0 μm and typically appear light brown or transparent.[6] A typical colony occurs individually or in pairs, with older cells forming chains of colonies.[6]
Genomics
Physiology
Cloacibacillus evryensis is a mesophile that can grow in temperature conditions of 20-50 °C and between pH values of 6.5-10.0.[6] The optimal growth conditions for C. evryenis are 37-40 °C and a pH 7.0, and the growth is enhanced by yeast extract.[6] Under the latter conditions, the doubling time of the organism on Casamino acid medium is 15 hours.[6] Cultures grown in Casamino medium between 0.1-2.0% weight per volume concentrations of NaCl observed growth up to 0.07% NaCl; at any higher concentration, growth is inhibited.[6]
