Cyanohydrin beta-glucosyltransferase
Class of enzymes
From Wikipedia, the free encyclopedia
Cyanohydrin beta-glucosyltransferase (EC 2.4.1.85) is an enzyme that catalyzes the chemical reaction:[1]
-4-hydroxymandelonitrile.svg)
| Cyanohydrin beta-glucosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.1.85 | ||||||||
| CAS no. | 55354-52-4 | ||||||||
| Databases | |||||||||
| BRENDA | enzyme data | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | enzyme entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| Rhea | reactions | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Tyrosine N-monooxygenase, the first enzyme in the pathway to dhurrin, converts L-tyrosine to (E)-(4-hydroxyphenyl)acetaldehyde oxime. Subsequently, (S)-4-hydroxymandelonitrile is formed by the action of 4-hydroxyphenylacetaldehyde oxime monooxygenase.[2]
Cyanohydrin beta-glucosyltransferase uses uridine diphosphate glucose (UDP-D-glucose) to add the glucoside component.[3][4] The enzyme is a glycosyltransferase, specifically one of the hexosyltransferases. The systematic name of this enzyme class is UDP-D-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase. Other names in common use include uridine diphosphoglucose-p-hydroxymandelonitrile, glucosyltransferase, UDP-glucose-p-hydroxymandelonitrile glucosyltransferase, uridine diphosphoglucose-cyanohydrin glucosyltransferase, uridine diphosphoglucose:aldehyde cyanohydrin, beta-glucosyltransferase, UDP-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase, UGT85B1, and UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase.[5]
The enzyme from Sorghum bicolor can act on a range of alcohols, not just the cyanohydrin group of 4-hydroxymandelonitrile.[6]
