Ent-kaurene oxidase

Ent-kaurene oxidase (EC 1.14.14.86, Formerly EC 1.14.13.78) is an enzyme with systematic name ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating).^[1][2][3] This enzyme catalyses the following chemical reaction

ent-kaur-16-ene + 3 NADPH + 3 H⁺ + 3 O₂ ${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-oate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) ent-kaur-16-ene + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-ol + NADP⁺ + H₂O

(1b) ent-kaur-16-en-19-ol + NADPH + H⁺ + O₂ ${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-al + NADP⁺ + 2 H₂O

(1c) ent-kaur-16-en-19-al + NADPH + O₂ ${\displaystyle \rightleftharpoons }$ ent-kaur-16-en-19-oate + NADP⁺ + H₂O

Ent-kaurene oxidase is a cytochrome p450 class enzyme.^[4]