Human Proteinpedia
From Wikipedia, the free encyclopedia
Human Proteinpedia, which is closely associated with Institute of Bioinformatics (IOB), Bangalore and Johns Hopkins University, is a portal for sharing and integration of human proteomic data.[1][2] It allows research laboratories to contribute and maintain protein annotations. Human Protein Reference Database (HPRD) integrates data, that is deposited in Human Proteinpedia along with the existing literature curated information at the context of an individual protein.[3][4] In essence, researchers can add new data to HPRD by registering to Human Proteinpedia. The data deposited in Human Proteinpedia is freely available for download. Emphasizing the importance of proteomics data disposition to public repositories, Nature Methods recommends Human Proteinpedia in their editorial.[5] More than 70 labs participate in this effort.
Data pertaining to post-translational modifications, protein–protein interactions, tissue expression, expression in cell lines, subcellular localization and enzyme substrate relationships can be submitted to Human Proteinpedia.
Experimental platforms
Protein annotations present in Human Proteinpedia are derived from a number of platforms such as
- Co-immunoprecipitation and mass spectrometry-based protein–protein interaction
- Co-immunoprecipitation and Western blotting based protein–protein interaction
- Fluorescence based experiments
- Immunohistochemistry
- Mass spectrometric analysis
- Protein and peptide microarrays
- Western blotting
- Yeast two-hybrid based protein–protein interaction
This portal that allows adding of protein information was developed as a collaborative effort between the laboratory of Dr. Akhilesh Pandey at Johns Hopkins University and the Institute of Bioinformatics.