Siegelová studied cuneiform and prehistory at Charles University, Prague, and joined the Náprstek Museum after graduating in 1967.[1] In 1971 she published her dissertation on two Hittite mythological-literary texts, the tale of Appu and his sons (CTH 360) and the Song of Ḫedammu (CTH 348), as part of the series Studien zu den Boğazköy-Texten.[2][3] In the same year, she became curator of the Ancient Near East collection in the Náprstek Museum.[1] In 1979, Siegelová became the director of the museum, a position she held until 2008.[1]
In 1996, Siegelová published a systematic bibliography of Hittitology, 1915-1995, which her husband, the Hittitologist Vladimír Souček, had also contributed to prior to his death.[4] Siegelová subsequently continued to contribute to Hittite bibliographical projects, notably the Systematic Bibliography[5] and the bibliographical database[6] hosted by the Hethitologie Portal Mainz.[7] A festschrift for Siegelová, Audias fabulas veteres. Anatolian Studies in Honor of Jana Součková-Siegelová, was published in 2016.[1]
