Karnovsky fixative
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Karnovsky fixative, developed by the pathologist Morris J. Karnovsky (1926–2018), is a fixative for electron microscopy.[1]
The stock solution for Karnovsky fixative is as follows:[1]
- 2.0 g paraformaldehyde
- 25 ml distilled water
- 1M sodium hydroxide 2 to 4 drops
- 50% glutaraldehyde 5.0 ml
- 0.2M cacodylate buffer, pH 7.4, 20.0 ml
Mix the paraformaldehyde with 25 ml of distilled water in a 125 ml Erlenmeyer flask. Heat to 60 °C on a stir plate. When moisture forms on the sides of flask, add sodium hydroxide and stir until the solution clears. Cool solution under the faucet. Filter, add glutaraldehyde and 0.2M buffer, pH range 7.2 to 7.4.