Metaproteomics

The term "metaproteomics" was proposed by Francisco Rodríguez-Valera to describe the genes and/or proteins most abundantly expressed in environmental samples.^[3] The term was derived from "metagenome". Wilmes and Bond proposed the term "metaproteomics" for the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time.^[4] At the same time, the terms "microbial community proteomics" and "microbial community proteogenomics" are sometimes used interchangeably for different types of experiments and results.

Metaproteomics allows for scientists to better understand organisms' gene functions, as genes in DNA are transcribed to mRNA which is then translated to protein. Gene expression changes can therefore be monitored through this method. Furthermore, proteins represent cellular activity and structure, so using metaproteomics in research can lead to functional information at the molecular level. Metaproteomics can also be used as a tool to assess the composition of a microbial community in terms of biomass contributions of individual members species in the community and can thus complement approaches that assess community composition based on gene copy counts such as 16S rRNA gene amplicon or metagenome sequencing.^[5]

The first proteomics experiment was conducted with the invention of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE).^[6][7] The 1980s and 1990s saw the development of mass spectrometry and mass spectrometry based proteomics. The current proteomics of microbial community makes use of both gel-based (one-dimensional and two-dimensional) and non-gel liquid chromatography based separation, where both rely on mass spectrometry based peptide identification.

While proteomics is largely a discovery-based approach that is followed by other molecular or analytical techniques to provide a full picture of the subject system, it is not limited to simple cataloging of proteins present in a sample. With the combined capabilities of "top-down" and "bottom-up" approaches, proteomics can pursue inquiries ranging from quantitation of gene expression between growth conditions (whether nutritional, spatial, temporal, or chemical) to protein structural information.^[1]

A metaproteomics study of the human oral microbiome found 50 bacterial genera using shotgun proteomics. The results agreed with the Human Microbiome Project, a metagenomic based approach.^[8]

Similarly, metaproteomics approaches have been used in larger clinical studies linking the bacterial proteome with human health. A recent paper used shotgun proteomics to characterize the vaginal microbiome, identifying 188 unique bacterial species in 688 women profiled.^[9] This study linked vaginal microbiome groups to the efficacy of topical antiretroviral drugs to prevent HIV acquisition in women, which was attributed to bacterial metabolism of the drug in vivo. In addition, metaproteomic approaches have been used to study other aspects of the vaginal microbiome, including the immunological and inflammatory consequences of vaginal microbial dysbiosis,^[10] as well as the influence of hormonal contraceptives on the vaginal microbiome.^[11]

Aside from the oral and vaginal microbiomes, several intestinal microbiome studies have used metaproteomic approaches. A 2020 study done by Long et al. has shown, using metaproteomic approaches, that colorectal cancer pathogenesis may be due to changes in the intestinal microbiome. Several proteins examined in this study were associated with iron intake and transport as well as oxidative stress, as high intestinal iron content and oxidative stress are indicative of colorectal cancer.^[12]

Another study done in 2017 by Xiong et al. used metaproteomics along with metagenomics in analyzing gut microbiome changes during human development. Xiong et al. found that the infant gut microbiome may be initially populated with facultative anaerobes such as Enterococcus and Klebsiella, and then later populated by obligate anaerobes like Clostridium, Bifidobacterium, and Bacteroides. While the human gut microbiome shifted over time, microbial metabolic functions remained consistent, including carbohydrate, amino acid and nucleotide metabolism.^[13]

A similar study done in 2017 by Maier et al. combined metaproteomics with metagenomics and metabolomics to show the effects of resistant starch on the human intestinal microbiome. After subjects consumed diets high in resistant starch, it was discovered that several microbial proteins were altered such as butyrate kinase, enoyl coenzyme A (enoyl-CoA) hydratase, phosphotransacetylase, adenylosuccinate synthase, adenine phosphoribosyltransferases, and guanine phosphoribosyltransferases. The human subjects experienced increases in colipase, pancreatic triglyceride lipase, bile salt-stimulated lipase abundance while also experiencing a decrease in α-amylase.^[14] Metaproteomics has also been used to understand the human-microbiome interactions that may underlie cardiovascular health. Using machine learning, a 2025 study by Yang et al. showed that human and microbial proteins could identify those at high-risk of cardiovascular disease in healthy and heart failure cohorts.^[15] These were proteins were primarily associated with intestinal inflammation and production of short-chain fatty acids.

Overall, metaproteomics has gained immense popularity in human intestinal microbiome studies as it has led to important discoveries in the health field.^{[citation needed]}