Optimized Multicolor Immunofluorescence Panel

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Optimized Multicolor Immunofluorescence Panel, abbreviation OMIP, was a series of standardized multi-color flow cytometry panel launched by International Society for Advancement of Cytometry (ISAC), published in the form of columns in Cytometry Part A.^[1]^[2]^[3] The first two OMIP articles were published in September 2010 issue of this journal.^[4]

Purpose

The goals of OMIPs are:

to alleviate the development time for researchers who wish to use the same (or highly similar) panels
to provide a starting point for the creation of novel OMIPs, and
to provide a mechanism for attribution to the developers of the panel via citation of the publication.^[1]

List

More information No., No. of colors ...

No.	No. of colors	Purpose	Species	Cell types	Paper	Year	Cytometry
OMIP-001	16	T-cell cytokine production after in vitro stimulation and phenotyping of cytokine-producing T-cells	Human	PBMC	^[5]^[4]	2010	BD LSR II
OMIP-002	16	Specificity and phenotype of CD8+ T-cells	Human	PBMC, bone marrow mononuclear cells	^[6]^[4]	2010	BD LSR II
OMIP-003	12	Phenotyping memory B cells	human	PBMC, bone marrow, and tonsil mononuclear cell	^[7]	2011	BD LSR II
OMIP-004	15	Determine Treg levels and to provide an in depth characterization of Tregs	Human	PBMC, ACK-lysed blood	^[8]	2012	BD LSRFortessa
OMIP-005	11	T cell cytokine production and memory phenotype	Rhesus macaque	PBMC	^[9]	2012	BD LSR II
OMIP-006	9	Enumerating and phenotyping of T regulatory cells and subsets	Human	PBMC	^[10]	2012	BD LSR II
OMIP-007	14	Characterize the phenotype, maturation, and activation of NK cells	Human	PBMC	^[11]	2012
OMIP-008	11	T cell cytokine production following in vitro stimulation	Human	PBMC, T cell clones/lines, tumor-resident T cell lines	^[12]	2012
OMIP-009	10	phenotype and function of CD4+ and CD8+ T-cells	Human	PBMC	^[13]	2012
OMIP-010	10	immunophenotyping of small hematopoietic cell	Human	Cerebrospinal fluid, bone marrow, peripheral blood	^[14]	2012
OMIP-011	8	Characterization of CECs in peripheral blood	Human	Whole blood, RBC-lysed and washed	^[15]	2012
OMIP-012	9	Human leukocyte reconstitution and phenotype	Humanized mouse	Whole blood, Ficoll-separated PBMC, splenocytes	^[16]	2012
OMIP-013	13	T-cell differentiation	Human	PBMC	^[17]	2012
OMIP-014	12	T-cell cytokine production and function after in vitro stimulation	Human	Cryopreserved PBMC	^[18]	2012
OMIP-015	13	T-reg characterization and T-cell activation	Human	PBMC	^[19]	2013
OMIP-016	10	Quality and phenotype of antigen-specific CD4 and CD8 T cells	Cynomolgus macaque or Human	Fresh or cryopreserved PBMCs	^[20]	2013
OMIP-017	13	CD4+ helper T-cell subsets (Th1, Th2, Th9, Th17, Th17Th1, Th22 and TFH)	Human	PBMC	^[21]	2013
OMIP-018	15	Phenotype of CD4 T cell subsets via chemokine receptor expression	Human	Fresh PBMC, Bone marrow mononuclear cells	^[22]^[23]	2013
OMIP-019	14	γδT-cells, iNKT-cells, haematopoietic precursors	Human	PBMC	^[24]	2013
OMIP-020	12	γδ T-cell differentiation phenotype	Human	PBMC	^[25]	2014
OMIP-021	14	NKT cells, γδ T-cells, MAIT cells, Memory T cell subsets	Human	PBMC	^[26]	2014
OMIP-022	14	T-cell phenotyping, memory categorization, cytokine production, and function following in vitro stimulation	Human	Cryopreserved PBMC (adult and infant)	^[27]	2014
OMIP-023	10	In-depth phenotyping of the main leukocyte subtypes in one run	Human	Fresh EDTA-treated peripheral blood, RBC-lysed and Fresh EDTA-treated Cord-blood	^[28]	2014
OMIP-024	18	Characterize the phenotype of PBMC leukocyte subsets	Human	Cryopreserved PBMC from adults and infants	^[29]	2014
OMIP-025	16	Characterization of antigen-specific T cells, TFH-like cells and NK cells	Human	Cryopreserved PBMC	^[30]	2014
OMIP-026	14	Phenotype of B and plasma cells	Rhesus macaque, African green monkey	Fresh whole blood, PBMCs, mononuclear cells of bone marrow, lymph node, spleen, tonsil	^[31]	2015
OMIP-027
OMIP-028
OMIP-029
OMIP-030
OMIP-031
OMIP-032					^[32]
OMIP-033
OMIP-034
OMIP-035
OMIP-036
OMIP-037
OMIP-038
OMIP-039
OMIP-040
OMIP-041
OMIP-042
OMIP-043
OMIP-044
OMIP-045
OMIP-046
OMIP-047
OMIP-048
OMIP-049	19	Subset analysis of hematopoiesis and hematopoietic disease	Human	Any source containing human myeloid cells	^[33]	2018	BD LSR II
OMIP-050	28				^[34]
OMIP-051
OMIP-052
OMIP-053
OMIP-054
OMIP-055
OMIP-056
OMIP-057
OMIP-058
OMIP-059
OMIP-060
OMIP-061
OMIP-062	14	Myeloid and innate/adaptive lymphoid comprehensive immunophenotyping	Human	Whole peripheral blood, cord blood, PBMCs, nasal aspirate, and tracheal aspirate samples	^[35]	2019	BD LSRFortessa
OMIP-063
OMIP-064
OMIP-065
OMIP-066
OMIP-067
OMIP-068
OMIP-069
OMIP-070
OMIP-071
OMIP-072
OMIP-073
OMIP-074
OMIP-075
OMIP-076
OMIP-077
OMIP-078
OMIP-079
OMIP-080
OMIP-081
OMIP-082	25
OMIP-083	18
OMIP-084	28
OMIP-085	8
OMIP-086	22
OMIP-087
OMIP-088
OMIP-089	8
OMIP-090	18
OMIP-091	27
OMIP-092	6
OMIP-093	41
OMIP-094	24
OMIP-095
OMIP-096
OMIP-097
OMIP-098
OMIP-099
OMIP-100
OMIP-101	27	Broad immunophenotyping of leukocytes including myeloid (neutrophils, monocytes, mDCs) and lymphoid cell lineages (conventional T cells, DURT cells, γδT cells, B cells, NK cells, ILCs)	Human	Fresh or fixed, cryopreserved whole blood	^[36]	2024	BD FACSymphony A5

Optimized Multicolor Immunofluorescence Panel

Purpose

List

References

Related Articles

Related Articles

"OMIP-003: phenotypic analysis of human memory B cells"

"OMIP-004: in-depth characterization of human T regulatory cells"

"OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells"

"OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry"

"OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells"

"OMIP-014: validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining"

"OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells"

"OMIP-022: Comprehensive assessment of antigen-specific human T-cell functionality and memory"

"OMIP-024: pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples"

"OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining"

"OMIP-049: Analysis of Human Myelopoiesis and Myeloid Neoplasms"

"OMIP-062: A 14-Color, 16-Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples"