Oxalobacter paeniformigenes
Species of bacterium
From Wikipedia, the free encyclopedia
Oxalobacter paeniformigenes is a Gram negative, non-spore-forming, oxalate-degrading anaerobic bacterium that was first isolated from human fecal samples.[1] Similar to other species in the Oxalobacter genus, O. paeniformigenes uses oxalate as its primary carbon source.[1] O. paeniformigenes is negative for indole production and negative for sulfate and nitrate reduction.[2] Cells appear rod shaped, though occasionally present as curved, and do not possess flagella.[2]
| Oxalobacter paeniformigenes | |
|---|---|
Scientific classification  | |
| Domain: | Bacteria |
| Kingdom: | Pseudomonadati |
| Phylum: | Pseudomonadota |
| Class: | Betaproteobacteria |
| Order: | Burkholderiales |
| Family: | Oxalobacteraceae |
| Genus: | Oxalobacter |
| Species: | O. paeniformigenes |
| Binomial name | |
| Oxalobacter paeniformigenes Chmiel et al, 2022 | |
| Type strain | |
| Oxalobacter paeniformigenes OxGP1T | |
The Type strain, OxGP1, was isolated from Guinea pig cecal contents.[3]
Taxonomy
Oxalobacter paeniformigenes was originally thought to be a subgroup of Oxalobacter formigenes.[1] Based on fatty acid profile and oxc (oxalyl-CoA decarboxylase) gene analysis, O. paeniformigenes strain OxGP1 was considered a group II strain.[4][5][6] However, 16S ribosomal RNA sequencing placed strain OxGP1 into group I.[1][6]
Whole genome sequencing confirmed that O. paeniformigenes strain OxGP1 is a different species from O. formigenes and it was subsequently renamed.[2] The new species name paeniformigenes uses the parent species formigenes and adds the Latin prefix paeni meaning "almost",[7] owing to the observation that species is related to but distinct from the parent species, O. formigenes.[2]
Genome
The genome of O. paeniformigenes is approximately 1.9 Mb with a G+C content of approximately 53.8%.[2] O. paeniformigenes has a smaller genome with slightly higher G+C content compared to other Oxalobacter species.
Growth in culture
O. paeniformigenes grows in CO2-bicarbonate buffered oxalate media and is typically cultivated in anaerobic Hungate tubes or an anaerobic chamber.[1] Oxalate is supplemented at 20 – 100 mM (depending on desired cell density) and bacteria are grown at 37 °C for 24 – 48 hours.[1][2] Anaerobic roll tubes, which are opaque agar filled Hungate tubes are used for bacterial isolation.[1]