Platelet-poor plasma

1. Within 1 hour of blood collection, centrifuge capped citrate (blue top) tube for 15 minutes
2. Using a plastic transfer pipet, remove the top 3/4 of plasma and place it in a plastic centrifuge tube with cap.
3. Centrifuge the plasma (in the plastic centrifuge tube) for another 15 minutes.
4. Using a plastic transfer pipet, remove the top 3/4 into a plastic tube. Do not disturb the plasma in the bottom of the spun tube, where any residual platelets will be.
5. Aliquots with visible red cells or hemolysis (pink plasma) are not acceptable.
6. Freeze plasma immediately. Samples for most laboratory assays should be frozen within 4 hours of collection.

As a byproduct of PRP preparation, PPP may also find use in tissue engineering applications as an autologous degradable scaffold. This plasma portion is frequently discarded when used with PRP treatments.^[6]