Pyranose oxidase
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In enzymology, a pyranose oxidase (EC 1.1.3.10) is an enzyme that catalyzes the chemical reaction
- D-glucose + O2 2-dehydro-D-glucose + H2O2
| pyranose oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Pyranose oxidase tetramer, Trametes ochracea | |||||||||
| Identifiers | |||||||||
| EC no. | 1.1.3.10 | ||||||||
| CAS no. | 37250-80-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are D-glucose and O2, whereas its two products are 2-dehydro-D-glucose and H2O2.
Pyranose oxidase is able to oxidize D-xylose, L-sorbose, D-galactose,[1] and D-glucono-1,5-lactone, which have the same ring conformation and configuration at C-2, C-3 and C-4.[2]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is pyranose:oxygen 2-oxidoreductase. Other names in common use include glucose 2-oxidase, and pyranose-2-oxidase. This enzyme participates in pentose phosphate pathway. It employs one cofactor, FAD.
Structural studies
Use in biosensors
Pyranose oxidase produce higher power output than does glucose oxidase. It is also easier to express in high yields using E. coli.[1]
