Santo Loku Pio attended his primary school in Kworijic before joining Minor Seminary of Saint Mary in Juba and then attended St Mary's Secondary School, receiving the Sudan Certificate.[3] He was ordained Priest of Juba on 7 Jan. 2001 and served for 17 years and was consecrated as bishop on 20 Feb. 2011.[4] He was appointed auxiliary bishop of Juba and titular bishop of Equizetum on 27 Nov. 2010.[5]