Ski complex

Helicase activities are initiated by the N-terminal arm and the Ski2 insertion domain.^[2] In yeast, the complex guides RNA molecules to the exosome complex for degradation via a fourth protein, called Ski7, which contains a GTPase-like protein.^[3] Ski7 involves the 3’ to 5’ degradation of RNA through two different pathways, 3’ poly(A) tail shortening and the binding of the Ski2, Ski3, and Ski8 tetramer and the exosome.^[1]

Degradation of the 3' mRNA overhang occurs by association with the 80s ribosome. The 3' end of the mRNA is threaded through the ribosome to Ski2, preparing it for the degradation process. ^[4]

Biochemical studies also show that the Ski complex can thread RNA through the exosome complex, thereby coupling the Ski2 protein helicase function with the exoribonuclease activity, leading to degradation of the RNA strand.^[2]

• TRAMP complex
• Non-stop decay
• Nonsense mediated decay