Smoothened agonist

CAS Number

• 912545-86-9 ^Y
  364590-63-6 (hydrochloride)

PubChem CID

• 5284330

ChemSpider

• 21541484 ^Y

UNII

• 4XN97SZK4J

Smoothened agonist

Identifiers
IUPAC name 3-chloro-N-[(1r,4r)-4-(methylamino)cyclohexyl]-N-[3-(pyridin-4-yl)benzyl]benzo[b]thiophene-2-carboxamide
CAS Number	912545-86-9 Y 364590-63-6 (hydrochloride)
PubChem CID	5284330
ChemSpider	21541484 Y
UNII	4XN97SZK4J
ChEBI	CHEBI:138438
CompTox Dashboard (EPA)	DTXSID80415294
Chemical and physical data
Formula	C28H28ClN3OS
Molar mass	490.06 g·mol−1
3D model (JSmol)	Interactive image
SMILES ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51
InChI InChI=1S/C28H28ClN3OS/c1-30-22-9-11-23(12-10-22)32(28(33)27-26(29)24-7-2-3-8-25(24)34-27)18-19-5-4-6-21(17-19)20-13-15-31-16-14-20/h2-8,13-17,22-23,30H,9-12,18H2,1H3/t22-,23- Key:VFSUUTYAEQOIMW-YHBQERECSA-N

Smoothened agonist (SAG) was one of the first small-molecule agonists developed for the protein Smoothened,^[1] a key part of the hedgehog signaling pathway, which is involved in brain development as well as having a number of other functions in the body.

Smoothened agonist has been shown to aid proliferation and survival of developing neurons,^[2] and prevent drug-induced brain injury.^[3] When injected into the cerebellum of newborn mice with an induced Down syndrome-like condition, Smoothened agonist was able to stimulate normal cerebellum development, resulting in significant behavioural improvement once the mice had grown to adulthood.^[4]

Smoothened Agonist was capable of inducing androgen production in both prostate and bone stromal cells that was significantly greater than even similarly treated prostate cancer cells.^[5]

The substance has been used as part of a chemical cocktail to turn old and senescent human cells back into young ones (as measured by transcriptomic age), without turning them all the way back into undifferentiated stem cells.^[6]