Talk:Image Cytometry Standard

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Please find here a request to edit the Image Cytometry Standard page with an adjusted article underneath. This version contains up-to-date information separating the ics format and libics library (which is the open source implementation of the ics format). A dead link was removed, main text was adjusted to be more readable, internal links were added to some terms, and external links referencing the library implementation were added. LookedOutOfTheWindow (talk) 10:17, 20 February 2026 (UTC)


Quick facts Image Cytometry Standard, Filename extensions ...
Image Cytometry Standard
Filename extensions
.ics, .ids
Size limitationNo limit
Type of formatImage file formats
CompressionLossless
Open format?Yes
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Quick facts libics, Original authors ...
libics
Original authorsCris Luengo, Bert Gijsbers, Fons Laan, Frank de Jong, Peter Verveer, David Svoboda, Glenn Pierce, Damir Sudar, Geert van Kempen, Jan Jitze Krol, Chiel Baarslag, Fons Laan and Hans van der Voort
DeveloperScientific Volume Imaging B.V.
Initial releaseJune 7, 2005; 20 years ago (2005-06-07)[1]
Written inC, with support for C++ and MATLAB
LicenseGNU Lesser General Public License version 2.1
Websitesvi-opensource.github.io/libics/
Repositorygithub.com/svi-opensource/libics
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The Image Cytometry Standard (ICS) is a digital multidimensional image file format used in life sciences microscopy. It can store not just the image data, but also the microscopic parameters and settings used during the image acquisition.

ICS was first proposed in: P. Dean, L. Mascio, D. Ow, D. Sudar, J. Mullikin, Proposed standard for image cytometry data files, Cytometry, n. 11, pp. 561–569, 1990 .

The original ICS version 1 file format used two separate files: a text header file with .ics extension and another much larger .ids file, that contains the actual image data. This allowed for compression of the data while leaving the header file accessible. On the other hand, the newer ICS version 2 file format uses only one single .ics file with both the header and the image data together.

The .ics in the two-file format is a text file with fields separated by tabs, and lines ending with a newline character. In the newer ICS2 format this text header precedes the binary data.

The ICS format is capable of storing:

  • multidimensional and multichannel data.
  • images in 8, 16, 32 or 64 bit integer, 16, 32 or 64 bit floating point and floating point complex data.
  • all microscopic parameters directly relevant to the image formation
  • free-form comments.

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