Tryptic soy broth

Growth medium for bacteria From Wikipedia, the free encyclopedia

Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious).[1]

Uses

Sterility test medium in USP and EP as well as for inocula preparation for CLSI standards.[1]

TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.[2]

Preparation

To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25°C. The solution is then autoclaved for 15 minutes at 121°C.[3]

Tryptic Soy Agar contains per liter:

References

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