Yeast display
From Wikipedia, the free encyclopedia
Yeast display (or yeast surface display) is a protein engineering technique that uses the expression of recombinant proteins incorporated into the cell wall of yeast. This method can be used for several applications such as isolating and engineering antibodies[1] and determining host-microbe interactions. [2]
The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup and Eric T. Boder.[3] The technology was sold to Abbott Laboratories in 2001.[4]
How it works
A protein of interest is displayed as a fusion to the Aga2p protein on the surface of yeast. The Aga2p protein is used by yeast to mediate cell–cell contacts during yeast cell mating. As such, display of a protein via Aga2p likely projects the fusion protein from the cell surface, minimizing potential interactions with other molecules on the yeast cell wall[citation needed]. The use of magnetic separation and flow cytometry in conjunction with a yeast display library can be highly effective method to isolate high affinity protein ligands against nearly any receptor through directed evolution.[citation needed]