C10orf35

The physical properties of the c10orf35 protein were analyzed, and the molecular weight was predicted to be 13.2 kdal,^[4] and the isoelectric point was predicted to be 11.5.^[4] The properties of DUF4605 were also analyzed, and the molecular weight was predicted to be 6.0 kdal.^[4] The isoelectric point was predicted to be 6.9,^[4] which is significantly less basic than the full protein.

This protein includes a highly conserved region between amino acids 1 and 19 discovered by MSA.^[5] DUF 4605 is located between amino acids 62 and 92.^[1] The secondary structure of the c10orf35 protein was predicted to consist of a β-sheet between amino acids 3 and 5,^[6] an α-helix between 61 and 72,^[6] and a transmembrane α-helix between 92 and 112.^[7] The serine in position 62 can be phosphorylated to form a phosphoserine group.^[7]

The protein of interest has a similar, paralogous domain found in c4orf32,^[8] which is conserved in mammals, birds, very few reptiles, and one amphibian.^[9]

The protein coded for by c10orf35 has many orthologs found mainly in mammals, reptiles, few amphibians, one fish, and one invertebrate.^[10] Based on a multiple sequence alignment between representative orthologs, DUF 4605 and a second, highly conserved region were confirmed.^[5]

The c10orf35 protein has expression over the 75th percentile in the brain, spinal cord, and male reproductive organs.^[12] Within the male reproductive system, the RNA is found within the testis and prostate, while the protein is expressed in the epididymis.^[13] In the brain, the RNA is found in the cerebral cortex, while the protein is expressed ubiquitously throughout the brain.^[13]