Guanylate-binding protein

Structurally, GBPs consist of two domains: a globular N- terminal domain harboring the GTPase function, and an extended C- terminal helical domain.^[3][5] In addition, some members of the GBPs family harbor motifs (e.g., CaaX motifs) or additional domains that are thought to operate in protein-protein or protein-membrane interactions.^[6][7]

Some GBPs have exhibited the ability to bind not only guanosine triphosphate (GTP) to produce guanosine diphosphate (GDP) but also GDP to produce guanosine monophosphate (GMP) with equimolar affinity and high intrinsic rates of hydrolyzation. The physiological relevance of the GBP's GDPase activity might yield important insights to elucidate GBP-specific defensive profile versus other INF-induced GTPases(e.g.IRGs).^[7] Evidence has suggested GBPs as important players in a variety of disease conditions ranging from infectious and metabolic inflammatory diseases to cancer,^[3][8] In the context of cell protection against bacteria, early efforts conducting loss-function assays revealed a reduced host resistance to several pathogens when lacking GBPs.^[9] More recent studies have indicated that GBPs appear to be an agent that disturbs the structural integrity of bacteria, stimulates inflammasome signaling, forms complexes on pathogen-containing vesicles in infected cells, and fosters autophagy and oxidative mechanisms helping pathogen clearance.^[10][11]

Human GBP1 is secreted from cells without the need of a leader peptide, and has been shown to exhibit antiviral activity against Vesicular stomatitis virus and Encephalomyocarditis virus, as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to IFN-gamma.^[12]

GBP1, the most widely studied GBP, has been studied for its antimicrobial properties. It can effectively polymerize and target the lipopolysaccharide cell wall of gram-negative bacteria. In said bacteria type, the GBP1 polymer coating alters the lipopolysaccharide membrane, allowing access to other parts of the membrane by other innate antimicrobial agents within the cell to cause pathogen cell death.^[13] Besides detecting pathogens and causing bacterial cell lysis, GBP1 can also cause host-programmed cell death.^[14]

The GBP family of proteins is highly conserved among many different phyla.^[15] They are believed to be a shared gene family that is used to fight off mostly viral, parasitic, and bacterial infections.^[13][15] On that note, the expression of GBPs is noted to increase in humans once the body detects many different types of diseases ranging from the infections listed above to cancer.^[15] Due to the similarity between murine and human GBPs, mouse knockout studies have been utilized to investigate the different roles GBPs have in fighting off other diseases.^[15] These studies have confirmed that knocking out different GBPs has different effects on combating different infections.^[15]