LOC100287387

The human LOC100287387 gene is located on the minus strand of the q arm of chromosome 2 at 2q37.3.^[1] It overlaps the TWIST2 gene family on the plus strand of chromosome 2.^[2] The gene is formed by three exons, with two introns near the start codon.^[2]

There are no alternative splicings of the LOC100287387 gene (isoforms).^[2]

The LOC100287387 protein is formed by a 423 amino acid peptide sequence. The molecular mass is 44.4 kdal,^[5] and the isoelectric point is 10.77.^[6] There is a G-patch domain and a short domain of unknown function within the peptide sequence. There are many predicted modification sites within the amino acid sequence including cAMP- dependent phosphorylation sites (CampP), casein kinase 2 (CK2), and protein kinase C (PKC) phosphorylation sites, O-linked beta-N-acetylglucosamine sites, and a sumoylation site.^[3][7] The predicted secondary structure of the protein includes 8 short alpha-helices (15.6% of the protein), 14 short extended strands (12.1%), and the rest as random coils (72%).^[8]

In humans, there is low expression of LOC100287387 in all tissues. Highest expression is in the skin and central nervous system tissue such as the pons, superior cervical ganglion, trigeminal ganglion, and globus pallidus. However, expression was inconsistent among patients.^[9]

The promoter region of the LOC100287387 gene contains binding sites for many transcription factors which affect transcription levels of the gene. Within the promoter region, there are three TFIIB binding sites (initiates transcription), a cysteine-serine-rich nuclear protein 1 site (an activator), a Kruppel-like zinc finger protein 219 site (repressor), a stimulating protein 1 site (activator), and many more.^[10]