NlaIII
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NlaIII is a type II restriction enzyme isolated from Neisseria lactamica.[1] As part of the restriction modification system, NlaIII is able to prevent foreign DNA from integrating into the host genome by cutting double stranded DNA into fragments at specific sequences.[2] This results in further degradation of the fragmented foreign DNA and prevents it from infecting the host genome.[3]
NlaIII recognizes the palindromic and complementary DNA sequence of CATG/GTAC and cuts outside of the G-C base pairs. This cutting pattern results in sticky ends with GTAC overhangs at the 3' end.[4]
