2016 Santiago Challenger

Country	Player	Rank1	Seed
ARG	Horacio Zeballos	73	1
BRA	Thiago Monteiro	88	2
ARG	Renzo Olivo	105	3
BRA	Rogério Dutra Silva	107	4
BRA	João Souza	118	5
ARG	Guido Andreozzi	126	6
ARG	Leonardo Mayer	127	7
ARG	Nicolás Kicker	132	8

• ¹ Rankings are as of October 10, 2016.^[1]

The following players received wildcards into the singles main draw:

• Nicolás Jarry
• Jaume Munar
• Bastián Malla
• Marcelo Tomás Barrios Vera

The following player received entry into the singles main draw using a protected ranking:

• Fabiano de Paula

The following player entered as an alternate:

• Michael Linzer

The following players received entry from the qualifying draw:

• Andrea Collarini
• Daniel Dutra da Silva
• Juan Pablo Ficovich
• Juan Pablo Paz

The following players entered as lucky losers:

• Facundo Mena
• João Pedro Sorgi

• Máximo González def. Rogério Dutra Silva, 6–2, 7–6^(7–5).

• Julio Peralta / Horacio Zeballos def. Sergio Galdós / Máximo González, 6–3, 6–4.