2016 Trofeo Città di Brescia

Country	Player	Rank1	Seed
SVK	Lukáš Lacko	112	1
CZE	Lukáš Rosol	113	2
SUI	Marco Chiudinelli	116	3
RUS	Evgeny Donskoy	118	4
ARG	Leonardo Mayer	132	5
ESP	Enrique López Pérez	163	6
RUS	Alexander Kudryavtsev	173	7
SRB	Marko Tepavac	175	8

• ¹ Rankings are as of November 7, 2016.

The following players received wildcards into the singles main draw:

• Matteo Berrettini
• Tommy Robredo
• Stefano Travaglia
• Andrea Vavassori

The following player received entry into the singles main draw with a protected ranking:

• Blaž Kavčič

The following player received entry into the singles main draw as a special exempt:

• Kevin Krawietz

The following players received entry from the qualifying draw:

• Egor Gerasimov
• Marek Jaloviec
• Petr Michnev
• Lukas Rüpke

The following players received entry as lucky losers:

• Laurynas Grigelis
• Gianluca Mager

• Luca Vanni def. Laurynas Grigelis, 6–7^(5–7), 6–4, 7–6^(10–8).

• Mikhail Elgin / Alexander Kudryavtsev def. Wesley Koolhof / Matwé Middelkoop, 7–6^(7–4), 6–3.

• Official Website Archived 2014-11-29 at the Wayback Machine