2024 Challenger Ciudad de Guayaquil

Country	Player	Rank1	Seed
ARG	Francisco Comesaña	97	1
ARG	Federico Coria	101	2
COL	Daniel Elahi Galán	113	3
ARG	Román Andrés Burruchaga	129	4
ARG	Marco Trungelliti	135	5
ARG	Juan Manuel Cerúndolo	149	6
CZE	Vít Kopřiva	150	7
ARG	Federico Agustín Gómez	157	8

• ¹ Rankings are as of 21 October 2024.^[1]

The following players received wildcards into the singles main draw:

• Francisco Castro
• Marcos Lee Chan Baratau
• Ángel Véliz

The following player received entry into the singles main draw using a protected ranking:

• Nicolás Kicker

The following players received entry into the singles main draw as alternates:

• Gastão Elias
• Renzo Olivo
• Franco Roncadelli

The following players received entry from the qualifying draw:

• Alex Barrena
• Gerard Campaña Lee
• Mathys Erhard
• Garrett Johns
• Mariano Kestelboim
• Ryan Nijboer

• Federico Agustín Gómez def. Tomás Barrios Vera 6–1, 6–4.

• Karol Drzewiecki / Piotr Matuszewski def. Luís Britto / Marcelo Zormann 6–4, 7–6^(7–2).