2024 Challenger de Santiago

Country	Player	Rank1	Seed
CHI	Tomás Barrios Vera	103	1
PER	Juan Pablo Varillas	110	2
ARG	Camilo Ugo Carabelli	127	3
ARG	Facundo Bagnis	147	4
ARG	Román Andrés Burruchaga	159	5
ARG	Juan Manuel Cerúndolo	160	6
BOL	Hugo Dellien	161	7
ARG	Genaro Alberto Olivieri	177	8

• ¹ Rankings are as of 4 March 2024.^[1]

The following players received wildcards into the singles main draw:

• Ignacio Buse
• João Fonseca
• Matías Soto

The following player received entry into the singles main draw using a protected ranking:

• Paul Jubb

The following player received entry into the singles main draw as an alternate:

• Matheus Pucinelli de Almeida

The following players received entry from the qualifying draw:

• Valerio Aboian
• Gabi Adrian Boitan
• Gerard Campaña Lee
• Federico Agustín Gómez
• Mariano Kestelboim
• Orlando Luz

• Juan Pablo Varillas def. Facundo Bagnis 6–3, 6–2.

• Fernando Romboli / Marcelo Zormann def. Boris Arias / Federico Zeballos 7–6^(7–5), 6–4.