2025 Challenger Ciudad de Guayaquil

Country	Player	Rank1	Seed
CHI	Tomás Barrios Vera	111	1
PAR	Daniel Vallejo	169	2
LBN	Hady Habib	173	3
ECU	Álvaro Guillén Meza	200	4
PER	Gonzalo Bueno	206	5
MEX	Rodrigo Pacheco Méndez	209	6
BOL	Murkel Dellien	262	7
ARG	Federico Agustín Gómez	270	8
ECU	Andrés Andrade	283	9

• ¹ Rankings are as of 10 November 2025.^[1]

The following players received wildcards into the singles main draw:

• Emilio Camacho
• Mario André Galarraga
• Lucas Yúnez

The following players received entry into the singles main draw as alternates:

• Joaquín Aguilar Cardozo
• Ryan Dickerson
• Juan Estévez

The following players received entry from the qualifying draw:

• Federico Aguilar Cardozo
• Arklon Huertas del Pino
• Luis David Martínez
• Tomás Martínez
• Salvador Price
• Lucio Ratti

The following player received entry as a lucky loser:

• Juan Carlos Aguilar

• Daniel Vallejo def. Juan Pablo Varillas 7–5, 6–7^(7–9), 6–3.

• Alex Hernández / Rodrigo Pacheco Méndez def. Lucio Ratti / Victor Hugo Remondy Pagotto 7–5, 6–3.