2025 Santos Brasil Tennis Cup

Country	Player	Rank1	Seed
ECU	Álvaro Guillén Meza	205	1
PER	Juan Pablo Varillas	239	2
CHI	Matías Soto	240	3
PER	Gonzalo Bueno	248	4
ARG	Santiago Rodríguez Taverna	261	5
ARG	Juan Bautista Torres	273	6
BOL	Juan Carlos Prado Ángelo	291	7
ARG	Genaro Alberto Olivieri	302	8

• ¹ Rankings as of 21 April 2025.^[2]

The following players received wildcards into the singles main draw:

• Enzo Kohlmann de Freitas
• Bruno Malacarne
• Eduardo Ribeiro

The following player received entry into the singles main draw using a protected ranking:

• Blaise Bicknell

The following players received entry into the singles main draw as alternates:

• Hernán Casanova
• Gianluca Mager

The following players received entry from the qualifying draw:

• Arklon Huertas del Pino
• Conner Huertas del Pino
• Nicolás Kicker
• Bruno Kuzuhara
• Wilson Leite
• José Pereira

• Álvaro Guillén Meza def. Matheus Pucinelli de Almeida 6–3, 7–6^(14–12).

• Pedro Boscardin Dias / Gonzalo Villanueva def. Boris Arias / Federico Zeballos 6–2, 6–7^(3–7), [10–7].