2025 Open Bogotá

Country	Player	Rank1	Seed
COL	Nicolás Mejía[2]	227	1
CHI	Matías Soto	242	2
BRA	Karue Sell	299	3
BRA	Matheus Pucinelli de Almeida	306	4
ESP	Nicolás Álvarez Varona	314	5
BRA	Mateus Alves	322	6
BRA	Pedro Sakamoto	353	7
ARG	Renzo Olivo	354	8

• ¹ Rankings are as of 5 May 2025.^[3]

The following players received wildcards into the singles main draw:

• Samuel Heredia
• Salvador Price
• Miguel Tobón

The following player received entry into the singles main draw using a protected ranking:

• Arklon Huertas del Pino

The following players received entry from the qualifying draw:

• Mateo Barreiros Reyes
• Juan Sebastián Gómez
• Ignacio Monzón
• Daniel Antonio Núñez
• Pedro Rodrigues
• Johan Alexander Rodríguez

The following player received entry as a lucky loser:

• Tristan McCormick

• Patrick Kypson def. Pedro Sakamoto 6–1, 6–3.

• Luís Britto / Zdeněk Kolář def. Arklon Huertas del Pino / Conner Huertas del Pino 6–4, 7–6^(7–4).