2025 Challenger Concepción

Country	Player	Rank1	Seed
BRA	Thiago Monteiro	99	1
COL	Daniel Elahi Galán	110	2
ARG	Román Andrés Burruchaga	137	3
CHI	Tomás Barrios Vera	149	4
ARG	Facundo Mena	186	5
PAR	Daniel Vallejo	187	6
ARG	Andrea Collarini	198	7
USA	Emilio Nava	235	8

• ¹ Rankings are as of 17 March 2025.^[1]

The following players received wildcards into the singles main draw:

• Diego Dedura-Palomero
• Thiago Monteiro
• Nicolás Villalón

The following player received entry into the singles main draw as an alternate:

• Gonzalo Villanueva

The following players received entry from the qualifying draw:

• Valerio Aboian
• Pedro Boscardin Dias
• Conner Huertas del Pino
• Nicolás Kicker
• Vladyslav Orlov
• José Pereira

The following player received entry as a lucky loser:

• Yuki Mochizuki

• Emilio Nava def. Nicolás Kicker 6–1, 7–6^(7–3).

• Vasil Kirkov / Matías Soto def. Seita Watanabe / Takeru Yuzuki 6–2, 6–4.