2023 Open Rionegro

Country	Player	Rank1	Seed
COL	Nicolás Mejía	248	1
ARG	Santiago Rodríguez Taverna	253	2
ARG	Federico Delbonis	266	3
BRA	João Lucas Reis da Silva	273	4
ARG	Guido Andreozzi	286	5
CRO	Nino Serdarušić	293	6
USA	Patrick Kypson	328	7
AUS	Tristan Schoolkate	331	8

• ¹ Rankings are as of 19 June 2023.^[1]

The following players received wildcards into the singles main draw:

• Nicolás Mejía
• Johan Alexander Rodríguez
• Felipe Santamaría

The following players received entry into the singles main draw using protected rankings:

• Pedro Sakamoto
• Rubin Statham

The following players received entry from the qualifying draw:

• Juan Sebastián Gómez
• Aidan Mayo
• Tristan McCormick
• Calum Puttergill
• Matías Soto
• Nicolas Zanellato

The following player received entry as a lucky loser:

• Ignacio Monzón

• Patrick Kypson def. Benjamin Lock 6–3, 6–3.

• Juan Sebastián Gómez / Andrés Urrea def. Orlando Luz / Oleg Prihodko 6–3, 7–6^(12–10).