2023 Open Città della Disfida

Country	Player	Rank1	Seed
	Alexander Shevchenko	101	1
AUT	Filip Misolic	150	2
ITA	Franco Agamenone	166	3
FRA	Benoît Paire	170	4
ITA	Francesco Maestrelli	171	5
FRA	Laurent Lokoli	172	6
CZE	Zdeněk Kolář	174	7
ITA	Flavio Cobolli	177	8

• ¹ Rankings are as of 20 March 2023.^[1]

The following players received wildcards into the singles main draw:

• Federico Arnaboldi
• Flavio Cobolli
• Gianluca Mager

The following player received entry into the singles main draw as an alternate:

• James McCabe

The following players received entry from the qualifying draw:

• Jacopo Berrettini
• Edoardo Lavagno
• Stefano Napolitano
• Julian Ocleppo
• Gabriele Piraino
• Stefano Travaglia

The following player received entry as a lucky loser:

• Salvatore Caruso

• Shintaro Mochizuki def. Santiago Rodríguez Taverna 6–1, 6–4.

• Jacopo Berrettini / Flavio Cobolli def. Zdeněk Kolář / Denys Molchanov 1–6, 7–5, [10–6].