2023 Knoxville Challenger

Country	Player	Rank1	Seed
USA	Michael Mmoh	101	1
USA	Aleksandar Kovacevic	109	2
USA	Alex Michelsen	112	3
USA	Zachary Svajda	143	4
USA	Emilio Nava	148	5
FRA	Titouan Droguet	166	6
AUS	Adam Walton	174	7
CHN	Shang Juncheng	176	8

• ¹ Rankings are as of October 30, 2023.^[1]

The following players received wildcards into the singles main draw:

• Nishesh Basavareddy
• Strong Kirchheimer
• Johannus Monday

The following player received entry into the singles main draw as a special exempt:

• Aidan Mayo

The following players received entry from the qualifying draw:

• Murphy Cassone
• Sebastian Fanselow
• Evgeny Karlovskiy
• Cannon Kingsley
• Thai-Son Kwiatkowski
• Mats Rosenkranz

The following players received entry as lucky losers:

• Alexander Cozbinov
• Christian Harrison

• Alex Michelsen def. Denis Kudla 7–5, 4–6, 6–2.

• Cannon Kingsley / Luis David Martínez def. Mac Kiger / Mitchell Krueger 7–6^(7–3), 6–3.