2023 Santa Cruz Challenger

Country	Player	Rank1	Seed
ARG	Juan Manuel Cerúndolo	93	1
ARG	Genaro Alberto Olivieri	140	2
BOL	Hugo Dellien	147	3
ARG	Thiago Agustín Tirante	150	4
ARG	Francisco Comesaña	160	5
ARG	Mariano Navone	184	6
ITA	Luciano Darderi	185	7
ARG	Camilo Ugo Carabelli	202	8

• ¹ Rankings are as of 28 August 2023.^[1]

The following players received wildcards into the singles main draw:

• Juan Ignacio Londero
• Juan Carlos Prado Ángelo
• Matías Soto

The following player received entry into the singles main draw using a protected ranking:

• Pedro Sakamoto

The following players received entry from the qualifying draw:

• Pedro Boscardin Dias
• Orlando Luz
• Facundo Mena
• José Pereira
• Gonzalo Villanueva
• Marcelo Zormann

• Mariano Navone def. Francisco Comesaña 4–6, 7–5, 6–1.

• Boris Arias / Federico Zeballos def. Matías Soto / Gonzalo Villanueva 6–2, 4–6, [10–7].