2023 Challenger de Buenos Aires

Country	Player	Rank1	Seed
ARG	Federico Coria	83	1
ARG	Juan Manuel Cerúndolo	102	2
ARG	Facundo Díaz Acosta	106	3
CHI	Tomás Barrios Vera	108	4
CZE	Vít Kopřiva	114	5
CHI	Alejandro Tabilo	120	6
ARG	Francisco Comesaña	126	7
GBR	Jan Choinski	127	8

• ¹ Rankings are as of 25 September 2023.^[2]

The following players received wildcards into the singles main draw:

• Román Andrés Burruchaga
• Federico Delbonis
• Juan Pablo Ficovich

The following player received entry into the singles main draw as a special exempt:

• Andrea Pellegrino

The following player received entry into the singles main draw as an alternate:

• Guido Andreozzi

The following players received entry from the qualifying draw:

• Murkel Dellien
• Renzo Olivo
• João Lucas Reis da Silva
• Juan Bautista Torres
• Marco Trungelliti
• Gonzalo Villanueva

The following player received entry as a lucky loser:

• Gastão Elias

• Mariano Navone def. Federico Coria 2–6, 6–3, 6–4.

• Diego Hidalgo / Cristian Rodríguez def. Fernando Romboli / Marcelo Zormann 6–3, 6–2.