2025 Internazionali di Tennis Città di Trieste

Country	Player	Rank1	Seed
TPE	Tseng Chun-hsin	96	1
CHI	Tomás Barrios Vera	108	2
CZE	Dalibor Svrčina	114	3
USA	Tristan Boyer	120	4
ARG	Thiago Agustín Tirante	121	5
GEO	Nikoloz Basilashvili	126	6
ITA	Matteo Gigante	133	7
LTU	Vilius Gaubas	140	8

• ¹ Rankings are as of 30 June 2025.^[1]

The following players received wildcards into the singles main draw:

• Marco Cecchinato
• Federico Cinà
• Giulio Zeppieri

The following player received entry into the singles main draw as a special exempt:

• Stefano Travaglia

The following player received entry into the singles main draw through the Next Gen Accelerator programme:

• Arthur Géa

The following player received entry into the singles main draw as an alternate:

• Facundo Mena

The following players received entry from the qualifying draw:

• Andrea Collarini
• Matej Dodig
• Daniel Michalski
• Maxim Mrva
• Sumit Nagal
• Andrea Picchione

• Matej Dodig def. Thiago Agustín Tirante 6–3, 6–4.

• Jakub Paul / Matěj Vocel def. Robin Haase / Johannes Ingildsen 7–5, 6–1.