2025 Santa Cruz Challenger

Country	Player	Rank1	Seed
BOL	Murkel Dellien	220	1
BRA	Matheus Pucinelli de Almeida	282	2
ARG	Juan Bautista Torres	283	3
ARG	Genaro Alberto Olivieri	289	4
ARG	Renzo Olivo	304	5
BOL	Juan Carlos Prado Ángelo	310	6
ARG	Lautaro Midón	311	7
BRA	João Lucas Reis da Silva	313	8

• ¹ Rankings are as of 9 June 2025.^[1]

The following players received wildcards into the singles main draw:

• Facundo Bagnis
• Raúl García
• Federico Zeballos

The following player received entry into the singles main draw as an alternate:

• Tomás Farjat

The following players received entry from the qualifying draw:

• Luis Carlos Álvarez
• Diego Augusto Barreto Sánchez
• Santiago de la Fuente
• Lucas Gerch
• John Sperle
• Bautista Vilicich

The following player received entry as a lucky loser:

• Juan Manuel La Serna

• Alex Barrena def. Franco Roncadelli 5–7, 7–5, 6–3.

• Mariano Kestelboim / Gonzalo Villanueva def. Boris Arias / Federico Zeballos 6–3, 6–2.