2025 UniCredit Czech Open

Country	Player	Rank1	Seed
CZE	Jakub Menšík	19	1
CHI	Alejandro Tabilo	61	2
CZE	Vít Kopřiva	86	3
BOL	Hugo Dellien	90	4
TPE	Tseng Chun-hsin	94	5
KAZ	Alexander Shevchenko	103	6
CZE	Dalibor Svrčina	127	7
HUN	Zsombor Piros	160	8

• ¹ Rankings are as of 26 May 2025.^[1]

The following players received wildcards into the singles main draw:

• Jakub Menšík^[2]
• Maxim Mrva
• Alejandro Tabilo

The following player received entry into the singles main draw as an alternate:

• Nikolás Sánchez Izquierdo

The following players received entry from the qualifying draw:

• Hynek Bartoň
• Petr Brunclík
• Jonáš Forejtek
• Norbert Gombos
• Miloš Karol
• Lukáš Pokorný

• Hugo Dellien def. Tseng Chun-hsin 6–3, 6–4.

• Petr Nouza / Patrik Rikl def. Lukáš Pokorný / Dalibor Svrčina 4–6, 6–3, [10–4].