2025 Challenger de Villa María

Country	Player	Rank1	Seed
USA	Emilio Nava	101	1
BOL	Hugo Dellien	122	2
ITA	Francesco Maestrelli	160	3
ARG	Federico Agustín Gómez	185	4
ARG	Genaro Alberto Olivieri	206	5
PER	Gonzalo Bueno	209	6
ARG	Alex Barrena	225	7
BRA	João Lucas Reis da Silva	232	8

• ¹ Rankings are as of 8 September 2025.^[3]

The following players received wildcards into the singles main draw:

• Fernando Cavallo
• Juan Manuel La Serna
• Juan Bautista Torres

The following players received entry into the singles main draw as alternates:

• Mariano Kestelboim
• Lorenzo Joaquín Rodríguez

The following players received entry from the qualifying draw:

• Facundo Bagnis
• Hernán Casanova
• Santiago de la Fuente
• Lautaro Agustín Falabella
• Facundo Juárez
• Carlos María Zárate

• Emilio Nava def. Alex Barrena 6–3, 6–3.

• Guillermo Durán / Mariano Kestelboim def. Daniel Dutra da Silva / Gonzalo Villanueva 6–4, 6–2.